Agarose gel isoelectric focusing.

Two-dimensional gel electrophoresis, isoelectric focusing and agarose gel electrophoresis in the diagnosis of multiple sclerosis.

Two dimensional gel electrophoresis (2-DE), isoelectric focusing (IEF) and agarose gel electrophoresis (AGE) were used to examine cerebrospinal fluid (CSF) and sera from 22 patients with confirmed multiple sclerosis, 11 patients with probable multiple sclerosis and 20 control patients with non-inflammatory neurological diseases of the central nervous system (CNS). All of the 22 patients with co...

Two−dimensional separation of human plasma proteins using agarose gel isoelectric focusing followed by SDS capillary electrophoresis

Two−dimensional polyacryiamide gel electrophoresis (2−D PAGE) has been widely used for protein separation because of its high resolution; more than one thousand proteins or polypeptides can be separated according to the differences of their isoelectric point (pI) and molecular mass. However, 2−D PAGE involves many steps of manual handling, sample injection, setting gel molds in an electrophores...

Isoelectric focusing of proteins in rewetted ultrathin polyacrylamide gel layers.

Ultrathin layers of polyacrylamide gel bound to glass can be washed, air-dried, and stored for at least 1 year before rewetting in ampholyte solutions for isoelectric focusing. Short-term drying affects neither fluorescent banding of the ampholytes (not evident in conventional gels) nor resolution of complex protein mixtures while prolonged storage seems to have no deleterious effects. Layers a...

Isoelectric focusing.

Capillary Isoelectric Focusing

Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...